Exploring profilin

Profilin, and Vascular Diseases

Profilin for processivity

SNX1 chooses its home via two membrane-binding domains. One targeting domain is the PX domain, which is known to bind to the endosomal phosphoinositide, PI3P. The second is a BAR domain, which was shown to target a fly protein to highly curved membranes and tubulate them. Cullen’s group shows that these domains combine to put mammalian SNX1 on the tubular portion of early endosomes (which also ...

Profilin isoforms in Dictyostelium discoideum.

Eukaryotic cells contain a large number of actin binding proteins of different functions, locations and concentrations. They bind either to monomeric actin (G-actin) or to actin filaments (F-actin) and thus regulate the dynamic rearrangement of the actin cytoskeleton. The Dictyostelium discoideum genome harbors representatives of all G-actin binding proteins including actobindin, twinfilin, and...

Alternative splicing of the mouse profilin II gene generates functionally different profilin isoforms.

Profilins are a conserved family of proteins participating in actin dynamics and cell motility. In the mouse, two profilin genes are known. Profilin I is expressed universally at high levels, while profilin II is expressed mainly in the brain. Here we describe the occurrence of two mouse profilin II isoforms, A and B, which are derived by alternative splicing. They are identical through residue...

Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells

We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Müller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins fr...